"We need to make sure there is a thorough and transparent, credible investigation", Hale said, while touring the blast site on the final day of a visit to the crisis-hit country.
"We can never go back to an era in which anything goes at the port or the borders of Lebanon."
A huge stock of hazardous material abandoned in a warehouse at the port in the heart of the capital exploded on August 4, killing 177 people and devastating swathes of Beirut.
The hundreds of tonnes of ammonium nitrate had been left unsecured for several years, despite repeated warnings of the dangers it posed.
"Every state, every sovereign state, controls its ports and its borders thoroughly,” said Hale, US undersecretary for political affairs.
"I imagine all Lebanese would like... not to have the anything goes atmosphere.”
The disaster led to demands at home and abroad for an international investigation, calls that have been rebuffed by Lebanon’s political leaders, widely accused of negligence that led to the explosion.
"The FBI is arriving this weekend and they will be playing their role at the invitation of the Lebanese,” Hale said, adding they would help determine "what led to the circumstances of this explosion”.
The powerful Shiite group Hizbollah stands accused of wielding great influence over Beirut’s port and border posts.
The explosion reignited claims that Hizbollah, which is designated by Washington as a terrorist group, stored arms at the blast site.
The movement’s chief Hassan Nasrallah on Friday strongly denied the charges.
"We have nothing in the port: not an arms depot, nor a missile depot nor missiles nor rifles nor bombs nor bullets nor [ammoniu] nitrate,” he insisted.
Many Lebanese blame the blast on decades of corruption and negligence by Lebanon’s ruling class — consisting largely of ex- warlords from its 1975-1990 civil war.
The FBI personnel are to join other international experts already on the ground, including from France which has launched its own probe.
Lebanese authorities too have opened an enquiry, despite scepticism at home over the credibility of a state-led investigation.
Public Prosecutor Ghassan Oueidat has filed lawsuits against 21 suspects over the blast, 19 of whom are already in custody.
Judge Fadi Sawan, who was appointed on Friday to lead investigations, is to start interrogating suspects next week.